Search results for " parentage analysis"

showing 2 items of 2 documents

High-throughput 18K SNP array to assess genetic variability of the main grapevine cultivars from Sicily

2016

The viticulture of Sicily, for its vocation, is one of the most important and ancient forms in Italy. Autochthonous grapevine cultivars, many of which known throughout the world, have always been cultivated in the island from many centuries. With the aim to preserve this large grapevine diversity, previous studies have already started to assess the genetic variability among the Sicilian cultivars by using morphological and microsatellite markers. In this study, simple sequence repeat (SSR) were utilized to verify the true-to-typeness of a large clone collection (101) belonging to 21 biotypes of the most 10 cultivated Sicilian cultivars. Afterwards, 42 Organization Internationale de la Vigne…

0106 biological sciences0301 basic medicineGermplasmVitis vinifera LParentage analysiBiologyHorticulture01 natural sciences03 medical and health sciencesGeneticSingle nucleotide polymorphism (SNP)GeneticsCultivarGenetic variabilityParentage analysisMolecular BiologyBiotype identificationGeneticsGenetic diversityfood and beveragesForestryBiotype identification; Parentage analysis; Single nucleotide polymorphism (SNP); Vitis vinifera L; Forestry; Horticulture; Genetics; Molecular BiologySNP genotypingSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree030104 developmental biologyGenetic structureMicrosatellite010606 plant biology & botanySNP array
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Parentage verification of Valle del Belice dairy sheep using microsatellite markers

2008

The objectives of the present work were to evaluate polymorphism of microsatellite markers, develop a parentage test and estimate misidentification rate on Valle del Belice Sicilian dairy sheep breed. Polymorphism was evaluated from 184 randomly selected animals from 5 different flocks. A total of 21 microsatellite markers including CSRD247, ILST011, McM527, APO010, INRA132, DU194351, DU323541, FCB128, McM16, OarCP49, ILST087, DU223896, ILST005, TCRVB06, SPS113A, INRA063A, SPS115, DU206192, DU216028, BM827 and McM54 were amplified using three PCR reactions and fragment lengths determined in a single multiplex electrophoresis run. Markers were easily genotyped, very polymorphic and in Hardy-…

Settore AGR/17 - Zootecnica Generale E Miglioramento Geneticodairy sheep microsatellite markers parentage analysis
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